Coding
mamHIEJKL

Part:BBa_K590017:Design

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)

mamHIEJKL in vector pGA3K3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 8540
    Illegal XhoI site found at 89
    Illegal XhoI site found at 6375
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 2589
    Illegal NgoMIV site found at 3920
    Illegal NgoMIV site found at 6754
    Illegal NgoMIV site found at 8562
    Illegal NgoMIV site found at 8929
    Illegal AgeI site found at 5849
    Illegal AgeI site found at 6264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651
    Illegal BsaI site found at 1986
    Illegal BsaI site found at 3317
    Illegal BsaI.rc site found at 6947
    Illegal SapI site found at 825
    Illegal SapI site found at 2160
    Illegal SapI site found at 3491
    Illegal SapI.rc site found at 9208
    Illegal SapI.rc site found at 9529


Design Notes

Partial sequence confirmed, microscopy shows cells chain formation with this plasmid


Source

Made from Gibson Cloning Method. See [http://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Assembly Toolkit]

References